HER2 acts as a cell surface orphan receptor, and its high expression is one of the causes of breast cancer. For HER2-positive breast cancer patients, the HER2 antibody (ie Herceptin) is currently the only recommended biotherapeutic drug. The fluorescent probe obtained by coupling HER2 antibody with quantum dots is a commonly used fluorescent nanoprobe in HER2 positive breast cancer imaging research. The high fluorescence brightness of quantum dots and its fluorescence stability make the probe widely used. Molecular mechanisms and drug studies of in vitro and in vivo breast cancer.
The Jitendra Behari team at Nehru University in India injected the above-mentioned fluorescent probes (anti-HER2ab-QDs, 500 nM/100 μL) into Wistar rats (8-10 weeks) through the tail vein twice a day for 15 consecutive days. Then, the rat body weight, organ index, whole blood count, biochemical analysis, comet electrophoresis experiment, reactive oxygen species analysis, tissue section staining and apoptosis analysis were detected. The results showed that the fluorescent probe did not cause significant changes in whole blood count and biochemical indicators; no tissue damage and inflammatory reaction were caused, and no apoptosis was found in liver and kidney tissues. The above results suggest that antibody coating significantly reduces the adverse reactions of quantum dots, and anti-HER2ab-QDs have good biocompatibility as fluorescent nanoprobes.
The cytotoxicity of quantum dots is derived from the release of cadmium ions and the production of oxygen free radicals in their nanocrystalline cores. The most common strategy for weakening the toxicity of quantum dots is surface modification and coating with biocompatible molecules. For example, the nanocrystalline core is made of ZnS as the outer shell, and then coated with BSA, PEG, GSH, SiO2, etc., so that the quantum dot core is not easy. Oxidative degradation to reduce toxicity. Similarly, quantum dots coated with antibodies, peptides, small molecule compounds, etc., also help to improve their biocompatibility. The above research provides informative experimental data.
Source of the document:
Tiwari DK, Jin T, Behari J. Bio-distribution and toxicity assessment of intravenously anti-HER2 antibody conjugated CdSe/ZnS quantum dots in Wistar rats. Int J Nanomedicine. 2011;6:463-75.
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