Ganoderma lucidum cultivation techniques

Ganoderma lucidum, alias Ganoderma lucidum grass, fungus Ganoderma lucidum, ganoderma lucidum, etc., have nourishing strong, anti-inflammatory phlegm, cough and asthma and other effects. The production of Ganoderma lucidum in bottles (or bags) is easy to learn.

First, the preparation of nutrients

1 About 75% of wood chips, about 25% of wheat bran, about 0.2% of ammonium sulfate, 70% of water content of spices; 2 about 75% of wood chips, about 25% of wheat bran, 60% of water content of condiments; 3 about wood chips 50%, about 50% wheat bran, 0.1% urea, 60% moisture content of condiment, 40% cottonseed skin, 20% wheat bran, 1% sucrose, 60%-65% water content of mix, 5 corn shaft dregs 50%, about 30% of wood waste, about 20% of wheat bran, 60%-65% of water content of mix; 65% of poplar leaf, 25% of rice bran (or wheat bran), 60% of water content of condiment; 7 straw powder 45%, about 30% of wood chips, about 25% of wheat bran (or rice bran), and 60%-65% of water content of spices.

Second, loading and sterilization

After the broth was mixed, the mash was loaded for half an hour. Conventional plastic bags can hold dry materials from 0.25 kg to 0.3 kg. When hand-packed by hand, the material surface should be flat, then use a tapered wooden rod to make a 2.5-cm diameter vent hole from the middle of the material surface. The tampon should be tamped with a kraft paper. Such as bottling, available volume is 500 ml -1000 ml, diameter of 3.3 cm -4.6 cm wide mouth bottle or mushroom bottle, loading method basically the same bag method.

After filling, the bags (or bottles) are layered in a pot, sterilized at a pressure of 1.5 kilograms per square centimeter for 1 hour to 2 hours, or sterilized at normal pressure for 8 hours to 10 hours. It is required to be loaded on the same day and sterilized on the same day.

Third, inoculation and cultivation

The inoculation is best done in the inoculation box. If there is no inoculation box, it can be above the flame of the alcohol lamp or above the boiling water vapor. The inoculation room and inoculation tools should also be strictly disinfected. The staff wear masks and wash hands with soap 3 times. Take a piece of jujube-sized bacteria from the bottle with inoculated tweezers, quickly put it into the bottle (bag), and then plug the bottle mouth (or bag mouth) with a tampon and wrap the kraft paper and move it to the sterile culture room. Culture sterilization. Keeping the room temperature at 24°C-28°C, the mycelium grows into a bottle (bag) about 25 days after inoculation. In the bacteria-producing stage, management should be strengthened to prevent the contamination of bacteria, and those who have contamination with bacteria should be eliminated in time. When the bottles (bags) are filled with hyphae, the white buds of large fingertips appear on the surface of the culture material, namely the fruit body primordia. When the growth is close to the tampon, the tampon can be unplugged, the room temperature is controlled at 26°C-28°C, the relative humidity is increased to 80%-90%, scattered light is given, ventilation is performed every day, and the stipe is treated for about 20 days. The bottle (bag) mouth can be grown, and the caps are differentiated from the handle end. When the pale white or light yellow of the edge of the cap disappears, the edge of the cap will stop growing and become hard, and the color can be harvested when the color turns from brilliant to dark brown.

IV. Disease Prevention

During the production process, we must pay attention to the prevention of bacterial infections. The main bacteria are Penicillium, Mucor, and Rhizopus. Control methods: Strict asepsis during inoculation; Thorough disinfection of culture materials; Proper ventilation, low humidity; Mild infection with a sterile blade to scrape off local bacteria and surrounding bark; 75% alcohol cotton wool into the hole; serious pollution should be eliminated in time.

Cryopreservation Tube

The cryovial is made of medical polypropylene (PP) and is a disposable laboratory consumable specially used for storing biological samples. In the gaseous state of liquid nitrogen, it can withstand low temperature to
minus 187°C. The unique external rotation design avoids the possibility of cross-contamination. Silicone gasket inside the cap to avoid liquid leakage and ensure tightness even at cryogenic storage temperatures

The sealing property finally ensures the safety of the samples in the tube. Caps come with a variety of embeddable color codes for easy identification.

Cryopreservation Tube5 7 10ml 9

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