The "Research and Application of Biodegradation Technology of Pesticide Residues" undertaken by Nanjing Agricultural University realized the industrial production of pesticide residue decomposing bacteria. After three years of continuous use of the technology in Tuqiao Town, Jiangning District, Nanjing, the pollution-free rice produced by the company has been tested by the China Green Food Testing Center and has no pesticide residues. It has reached export standards and obtained a green food certificate. The pesticide residue microbial degradation technology is a new in-situ bioremediation technology with the independent intellectual property rights created by the research team led by Prof. Li Shunpeng of Nanjing Agricultural University. The project is aimed at severely exceeding pesticide residues in agricultural products and agro-ecological environment, and the serious degradation of the market competitiveness of agricultural products. Through the screening of high-efficiency pesticide residue-degrading strains, a large-scale fermentation of modern industry produces microbial agents. The microbial agent uses the enzymes produced by the microorganisms to apply the in situ bioremediation technology of pesticide residues to achieve the purpose of removing organic pollutants in soil, water, and agricultural products. This technology solves the problems of difficulty in physical and chemical treatment, high cost, and secondary pollution. At the same time, the project established a database of microbial germplasm resources for agro-environment and screened more than 20 strains of highly effective and safe degradation strains. The strains have superior performance, and the degradation rate of pesticide residues is as high as 95% to 99.8%. The strain itself is non-toxic, harmless, and free from secondary pollution. The effect of organophosphorus degrading bacteria on methyl parathion degradation is 100%. At the same time, 1 strain of degrading bacteria capable of degrading organochlorine and organophosphorus and simultaneously degrading more than 3 kinds of organophosphorus pesticides was constructed, and an engineering strain capable of efficiently degrading methyl parathion and carbofuran pesticides simultaneously was constructed. The researchers also conducted basic research on degrading strains, conducted a series of studies on metabolic pathways and material recycling of refractory organic compounds, molecular ecology studies of degrading bacteria, localization and cloning of degradative genes, expression of degradative enzyme genes, and enzymes. Scientific research, construction of genetically engineered bacteria and their environmental release safety assessment, stable expression of engineered bacteria, and high-efficiency expression studies have established a set of methods and methods for studying toxic and difficult-to-degrade organic compounds and microbial degradation, and established a comprehensive technology platform. The research level is at the international advanced level. At the same time, the project also conducted an ecological study of pesticide residue decomposing bacteria, and studied the ecological effects of pesticide-degrading bacteria using luminescent enzyme gene and green fluorescent protein (GFP) labeling method. This research is close to the international advanced level. The project is combined with the matching technology of fruit and vegetable fresh-cutting technology and detection technology to form a set of low-cost production and processing system for green agricultural products. At present, the technology has achieved remarkable economic and social benefits in the production of pollution-free agricultural products such as rice, tea, bamboo shoots and asparagus.
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