How to sterilize MH medium and how to use MH medium
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The MH referred to in the microorganism is the acronym for Mueller-Hinton, which refers to hydrolyzed casein. The MH medium refers to the hydrolyzed casein (Mueller-Hinton) medium, which can be configured as a MH blood agar medium for drug sensitivity experiments of streptococcus. This article mainly introduces how MH medium is most suitable for sterilization and how to use MH medium.
How to sterilize MH medium
The MH medium can be sterilized by moist heat sterilization at 115 degrees for 30 minutes.
How to use MH medium:
1. Weigh 38g of this product, add 1 L of distilled water or deionized water, stir and heat to boil until completely dissolved, dispense triangle flask, autoclave at 121 °C for 15 min, and set aside.
2. Preparation of test bacterial solution: Pick 3-5 morphologically similar colonies from the TSA plate to inoculate MHB or TSB medium. After incubation for 4-6 hours at 36±1 °C, the turbidity to 0.5 Mei unit .
3. Inoculation: Dilute the turbid liquid with a sterile cotton swab, gently pressurize the tube to remove excess bacteria, then gently apply the medium, and apply each dish three times. After each application Both plates need to be rotated 60 degrees, and then the next application. Each quality control strain is applied to three plates.
4. Patch: Place the plate after inoculation at room temperature for a while. After drying, use antiseptic tweezers to evenly spread the antibiotic paper on the surface of the plate agar. The center distance between the papers should not be less than 24mm, and the paper should not be away from the plate. Less than 15mm. Once the paper touches the surface of the agar, it can no longer move, so the drug has begun to spread.
5. Culture: After the paper is attached, in 15-30 minutes, the plate is turned over and placed at 36±1°C for 16-24 hours. Observe the results. It is not suitable to stack when the plate is cultured.
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