Plant Pharmacology
1. Antibacterial effect: Stem and leaf ethanol extracts have obvious inhibitory effect on Gram-positive bacteria in liquid test tubes. Humectone and elephonacin have significant inhibitory effects on Gram-positive bacteria such as Staphylococcus aureus, Streptococcus faecalis, Streptococcus pneumoniae, Diphtheria bacilli, Bacillus anthracis, Bacillus subtilis, and Bacillus cereus in vitro. The inhibitory concentration of hops was between 1:100000 and 1:1000000, and the bacteriostatic effect of shedtanone was weak. The antibacterial concentration of hops in vitro against Mycobacterium tuberculosis is 25 pg/ml. Infection with Mycobacterium tuberculosis H37Rvihvnu, intramuscular injection or lupulone for 30 consecutive days can make the liver, heart, lungs and spleen of infected mice dirty. The number of acid-fast bacilli in the lesions of the device was significantly reduced. The inhibitory effects of hopsulone and humulone on gram-negative bacteria, yeasts and fungi were weak or ineffective.
2. Other effects: humulone has a dinitrophenol-like effect on cats. After intravenous injection of 3 mg/kg, it can immediately increase the oxygen consumption by 1 time, and the occurrence of shortness of breath, followed by an increase in body temperature, due to excessive increase in body temperature. (45°C) to death. A large number of injections can also produce diabetes, hematuria. The effect on rabbits is far weaker.
3. Toxicity experiments: mice intramuscularly injected lupolone LD50 600mg/kg, died within l ~ 2h. Daily intramuscular injection of 60mg/kg for a total of four weeks, no obvious harmful effects, histopathological examination showed small leukocyte infiltration and degeneration of renal tubular lesions, 5% gum arabic suspension stomach 1500mg/kg, 1h mouse Half died.
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