With the development of molecular biology, genetic engineering research and application, centrifugal separation technology has also been replaced by several generations, from low-speed centrifuges , high-speed centrifuges , ultracentrifuges to ultra-speed refrigerated centrifuges , and ultra-large-capacity refrigerated centrifuges. . Here to introduce you to the common faults, troubleshooting methods and maintenance precautions of the centrifuge during clinical use, I hope to help your work.
1. In the process of use, the phenomenon that the motor can't start often occurs:
1. The main power indicator light is on. At this time, the degree of wear of the carbon brush should be checked. If the carbon brush wear exceeds one third of the total amount, a new carbon brush should be replaced in time.
2. When the main power indicator is off, check if the indicator fuse and the indoor distribution board fuse are blown. Also check whether the power line is in good contact. If the fuse is broken, replace the fuse and keep the circuit unblocked.
3, should also check the vacuum pump table and oil pressure indication value, if the oil pressure is too high and the host can not start, you must check whether the oil circuit is blocked, especially whether the throttle hole is smooth, if not smooth, it should be cleaned to make it smooth.
4. The bearing is damaged or the rotation is blocked. There is a lack of oil in the bearing or there is more dirt in the bearing, which causes the frictional resistance to increase. The motor does not reach the rated speed. The bearing should be cleaned or replaced in time.
5. There is a layer of oxide on the surface of the commutator. Even if the bump is uneven or the brush does not match the outer edge of the commutator, the speed can be reduced. The commutator and the brush should be cleaned to make the contact good.
6. If the rotor coil is short-circuited or broken, use a multimeter to check and re-wind the coil. The rotor can be used due to metal fatigue, overspeed, overstress, chemical corrosion, improper selection, use of intermediate head imbalance and temperature loss control, etc.; thus causing the centrifuge tube to rupture, sample leakage, rotor damage.
For the above situation, the operator is required to be proficient in the operation procedure, correctly select the appropriate centrifuge tube and centrifugal rotor, pay attention to strictly control each step of operation, minimize unnecessary damage caused by human beings, and use it in the safety factor and warranty period of the rotor. .
2. Power problems:
1. The power supply is blocked, which causes the refrigerator to start and the cooling effect is poor. One of the reasons is the same as the failure of the motor, and the power supply and fuse should be checked separately.
2, the voltage is too low, the safety device failure can also make the freezer can not start, the voltage is too low may be the grid voltage is low; the distribution board wiring too much; the power line is too long (ideal length is 3 meters). The power line capacity is not enough, which not only reduces the voltage, but also causes accidents. When the power supply voltage drops to 180-190VJ, the freezer cannot start, which affects the cooling effect.
3, the ventilation performance is not good, such as the instrument installed in direct sunlight or poor ventilation, the radiator is poor, or the radiator is covered with dust, it will affect the cooling effect.
4. The weight of the centrifuge tube is unbalanced and placed asymmetrically; there is foreign matter in the rotor hole to make the load unbalanced; the fixed nut on the upper end of the shaft is loose, the shaft is rubbed or bent; the rotor of the motor does not generate noise in the center of the magnetic field; the damage of the rotor itself can be The vibration of the body is severe and the noise is abnormal.
Most of the above are caused by incorrect operation. After finding out the cause, correct operation can eliminate the abnormal phenomenon. In the course of work, if any abnormality occurs, it should be stopped immediately, and it must not be forced to run, so as to avoid unnecessary losses.
3. The following is a brief introduction to the maintenance and precautions of the centrifuge:
1. The installation of the centrifuge should be placed according to the performance of the centrifuge. The general low-speed centrifuge (desktop) can be placed on a stable and sturdy table (the base is equipped with rubber feet, with the help of atmospheric force) The pressure and the weight of the instrument itself are close to the table top);
2. Large-capacity low-speed centrifuges and high-speed centrifuges and high-speed refrigerated centrifuges should be placed on a solid ground and placed horizontally. Before selecting a suitable rotor centrifuge, the load should be balanced. The smaller the weight error, the better. Otherwise, it will cause severe vibration and damage the centrifugal rotor and the shaft.
3. The centrifuge bracket centrifuge separation bracket must be placed after the centrifuge tube is operated, and the empty frame operation is strictly prohibited.
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