Determination of content
Take this product about 1.25g, accurately weighed, set the Erlenmeyer flask, add a freshly prepared ethanol potassium hydroxide titrant (0.5mol/L) 25ml, heated to reflux for 1 hour, rapidly evaporate the ethanol at a low temperature, residue heating 50ml of water is dispersed evenly, let cool, add 80ml of water and 50ml of magnesium sulfate solution (1.5→50), mix, let stand for 10 minutes, filter, filter residue is washed with 20ml of water, combine washing solution and filtrate, add hydrochloric acid to make acidic , Extraction with ether shaking 4 times, each time 40ml. The ether solutions were combined and extracted 5 times (20 ml, 20 ml, 10 ml, 10 ml, 10 ml) with shaking with sodium bicarbonate solution (1→20). Each separated aqueous solution was washed with 20 ml of the same ether. The aqueous solutions were combined and acidified by addition of hydrochloric acid. The mixture was extracted 4 times with chloroform (30 ml, 20 ml, 20 ml, 10 ml). Each time, the chloroform extract was filtered through a layer of absorbent cotton containing anhydrous sodium sulfate. When the combined filtrate evaporated to about 10 ml, stop evaporation, let it volatilize and dilute the solvent. The residue is dissolved with warm 10 ml of neutral ethanol (for phenol red indicator solution), allowed to cool, and add 2 to 3 drops of phenol red indicator solution. Titrate with sodium hydroxide titrant (0.1 mol/L). Each 1 ml of sodium hydroxide titrant (0.1 mol/L) corresponds to 14.82 mg of cinnamic acid (C9H8O2). This product contains total balsamic acid based on cinnamic acid (C9H8O2), not less than 28.5%.
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