l Cucumber (1) Production point interpolation method: Use black-skinned pumpkin as the rootstock, select the local varieties with strong affinity, good quality, and disease resistance to scion. The anvil and ear seeds must be disinfected and germinated before sowing. When winter and spring pods are grafted, black-skin squash should be sown for 2 to 3 days (with temperature-controlled seedlings). To be the second true leaf bud of rootstock seedlings, scion seedling diameter of 1cm, the best grafting period. Cut the growing point of the rootstock with a blade, and then use a bamboo needle (made with the same thickness of the scion as the scion) to insert 4~5mm vertically from the incision. The scion was cut off from the cotyledonary node 5 mm and cut off at an angle of 30 degrees from the hypocotyl, showing an elliptic section of 4 to 5 mm in length. Then remove the bamboo needle and insert the scion with the cotyledons into the needle hole so that the scion is matched with the rootstock and fixed with plastic clips. After purifying the water, place it in a small moist shed. In the following 3 days, the humidity was kept at 95%, the daytime temperature was 25 to 28°C, and the night time was 18 to 20°C. After 4 days, the ventilation was small and the membranes could be uncovered after 8 days. About 25 days into the clover can be planted. (2) Connect the rootstock in the nutrient bowl to the embryonic axis 5 to 10mm from the ion leaf section, and incline 6 to 10mm from the top to the bottom, and from 20 to 30mm below the scion cotyledon section to the bottom. Cut the 6-8 mm mouth (cut the seedlings without roots) and insert the spiked tongues into the cuts of the root stocks. Use a micro plastic clip to secure the joints and bury the scion roots in the soil. About 20 days, the base of the scion is cut off, and other management is basically the same as the line interpolation method. 2 Watermelon Watermelon in grafting and splicing applications widely. Plugs, docks and manages like cucumbers. Splicing: When the cotyledons of the rootstock are unfolded, the growth point is cut off and the cotyledon segments are divided into two vertical cuts. The scion is used as a single wedge surface to access from the cornice and fixed with a fixed object. The rootstocks grafted with watermelon are mainly melon melons, and watermelons in protected areas are promoted with new soil as rootstocks. Xintuzuo was linked to diploid watermelon and its survival rate was 53.5% to 96.5%. 3 The bitter gourd scion is (Yichun) 78-2, and the rootstock is the local meat loofah (early sowing 2 days). When the rootstock grows true leaves, the scions will be grafted when the leaves are in one. Cut the heart of the rootstock and cut a 6-8mm deep incision between the two cotyledons, insert the wedge-shaped scion, and bind and fix it. Within 3 days after grafting, full shading, 4 to 7 days from 9:00 am to 5:00 pm, followed by self-rooted seedling management 12 days later. 4 eggplant eggplant is mainly used to connect the method. Can be used Star, eggplant, belladonna, eggplant anvil 3, Congo eggplant, etc. as rootstock. The rootstock grafted with 5-6 leaves was the best. The survival rate of grafting with double wedge scion was very high. Rootstock 3 to 4 leaves, scion 3 leaves, scrotum mouth depth 8 ~ 10mm, scion to be inserted in the end, and then use grafting clips fixed. After the management is roughly the same as cucumber. 5 Tomato Tomatoes Wild tomato ch-2-26 is used as rootstock (sowing 7 to 10 days in advance). Tin powder and strong tomato are used as scion. When plugged in, the shade was applied for 10 days, then sprayed with water once every 3 hours during the day and planted after 35 days; when they were connected, the rootstock was planted in the nutrient bowl for about 30 days, and then the scion was transplanted into the rootstock for 1 week. Afterwards, the upper part of the rootstock was cut off after 5 to 7 days, and the base of the scion was cut off after 10 to 15 days, and then planted after 20 days of cultivation. Note: 1 cucumber winter pod grafted seedlings should pay special attention to insulation (under the seedbed set electric hotline), the first 7 days seedling shed temperature requirements during the day 25 ~ 30 °C, about 20 °C at night. The straight-in method scion cannot be inserted too deep. 2 eggplant spliced ​​scion should be inserted in the end will be better healing. 3 Grafting seedlings should be removed at any time. 4 When the grafted seedlings are planted, the soil should not be buried.
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Medical Indicator in the verification of the sterilization procedure,although the sterilization effect can be evaluated through the monitoring of certain parameters of the sterilization process,the degree of killing of the biological indicator is the most intuitive indicator for evaluating the effectiveness of a sterilization procedure.Commercially available standard biological indicators can be used,as can spores prepared with the most resistant micro-organisms isolated during daily production monitoring of contaminants.In the biological indicator verification test,the D value of the spore under the actual sterilization condition needs to be determined,and the spore purity and quantity are determined.When verifying,the microbial indicator of the biological indicator should be larger than the routine detection of microbial contamination,and the tolerance is strong to ensure that the sterilization procedure has greater safety.In the final sterilization method,biological indicators should be placed in different parts of the sterilizer.And avoid direct contact with the indicator to be sterilized items.The biological indicators are sterilized after being sterilized according to the set conditions,and cultured in a culture medium respectively to determine whether the spores in the biological indicator are completely killed.
Medical indicator in the sterilization biomonitoring of medical indicator,the standards
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