Blood collection methods and methods for large mice - venous blood
Blood collection of large mice--the way of blood collection, blood collection and advantages and disadvantages of venous blood
1. Tail vein: The blood collection volume of this pathway is generally 0.05-0.2ml, and the mouse is 0.05-0.1ml. It is suitable for blood glucose measurement and detection of indicators with very little serum requirement. The blood collection is small, but it is convenient and no anesthesia is needed. Hey~
2, jugular vein: the blood collection of this pathway is suitable for a single blood collection of more than 0.5ml; more than 0.1ml of the mouse, the master can not blindly wear blood, the new hand can be blinded under anesthesia or anatomical puncture; can also carry out more Puncture blood collection, or multiple blood collection and administration. No need for certain techniques in anesthesia, novices need to have a blood collection.
3, clavicular fossa vein: the same jugular vein, this route is characterized by wide blood vessels, blind wear is easy to locate.
4, femoral vein: suitable for anesthesia in the tube or blood collection, fine, need to contact the puncture technology.
5, right atrium: This approach is suitable for the master (嘿嘿..). Generally, the need for atrial blood is used in this way, the technical requirements are high, and the blood collection is large. It is easy to scratch the heart muscle and cause massive bleeding to die.
6, portal vein: this approach requires open surgery, direct under the collection, the portal vein is large, easy to puncture, blood collection.
7, abdominal main vein: This route is generally used for blood collection at the end of the semester, can collect the whole blood volume of animals.
8, the posterior iliac vein: this pathway is residual - tolerant, not recommended, and the collection is not pure blood, including arterial blood, venous blood, tissue fluid, the essence is a mixture, will seriously affect the accuracy of the detection indicators.
9, sublingual vein: this route uses a single blood collection with 0.1-0.5ml, easy to operate.
10, penile vein: This way, the amount of blood collected is small, generally rarely used.
. . . . . .
There are many ways to collect blood from venous blood. The above is often used, and many of the infrequently used small pieces are not described here one by one. . .
Of course, if the conditions permit, with ultrasound, many deep veins can be operated under ultrasound guidance~~~~
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