Today, I will introduce you to another sporty bacterium of Aeromonas , Aeromonas sobria ( As ).
Growth characteristics of Aeromonas
Aeromonas aeruginosa belongs to the family Vibrio, a genus of genus, a gram-negative bacterium, a short rod-shaped bacterium, a flagella on one side, a motility, no capsule, no spores. It grows well on ordinary agar medium. The colonies are white and light yellow translucent round, with neat edges, convex at the center, smooth surface, and β hemolysis on blood agar.
Pathogenicity of Aeromonas
Like Aeromonas hydrophila, mild Aeromonas is also widely distributed in fresh water, sewage, soil, human food chains, and water supply systems, causing humans, livestock, fish, and even amphibians and reptiles. Animals are also ill, which is also a human-animal-fish comorbidity pathogen. Mild Aeromonas is not mild at all, and its pathogenic ability is stronger than Aeromonas hydrophila. The main virulence factors are hemolysin, lysin, cytotoxic enterotoxin, lipase and protease. . Common diseases that can be caused by human infection include severe watery diarrhea or chronic diarrhea (lasting 10 days or more), cellulitis, sepsis, etc. It is very common that the disease has muscle lysis, gangrene, peritonitis, septic pneumonia, cholecystitis and bone marrow. Inflammation, etc.; mild Aeromonas often mixed with Aeromonas hydrophila to infect fish, edema of internal organs, tissue bleeding, ulceration and other symptoms, often causing heavy losses to the breeding industry.
Method for identifying Aeromonas hydrophila and Aeromonas
There are mainly microbial culture methods, immunology methods and molecular biology methods:
Microbial culture method, using selective medium to identify culture method and biochemical test identification method; through different biochemical reactions can identify the two;
Immunological methods mainly include enzyme-linked immunosorbent assay, monoclonal antibody-colloidal gold assay, etc.
Molecular biological methods include polymerase chain reaction, multiplex PCR, gene sequencing, and molecular labeling; dual PCR or multiplex PCR, using 16s RNA and virulence genes, such as glycerophospholipid cholesterol acyltransferase gene, gas lysin Gene, hemolysin gene, cytotoxic enterotoxin gene, thermostable cell excitatory enterotoxin gene, thermostable cell excitatory enterotoxin gene, amplification of two or more genes, can identify water vapor Aeromonas and mild Aeromonas.
Each of the above methods has its own characteristics, but in actual production applications, it exhibits long detection time, many operation steps, low accuracy, and insufficient sensitivity and specificity.
Xiaobian introduces a loop-mediated isothermal amplification (LAMP) method in molecular biology methods . This technique designs four specific primers based on six regions of the target gene, using a chain. The DNA polymerase was replaced and the amplification was continued at a constant temperature of 60-65 ° C for 45-60 min. The first successful application of the LAMP method for aquaculture animal pathogens is Delayed Edwards. At present, the loop-mediated isothermal amplification technique has been successfully applied to the detection of various Aeromonas, and the LAMP method can be used to identify the fascination quickly, conveniently, accurately and effectively. Aeromonas hydrophila and mild Aeromonas, without the need of professional background technicians and expensive molecular detection instruments, provide technical support for basic clinical diagnosis, and can be used as a screening method for large numbers of samples and preliminary screening judgment of results.
At present, the ZYD-S1 constant temperature fluorescent molecular detector introduced by Guangzhou Double Helix Gene Technology Co., Ltd. is equipped with a constant temperature fluorescent detection reagent with Aeromonas hydrophila and Aeromonas aeruginosa. The advantage is that the operation steps are few and simple. The amplification speed is fast, the time is short, and the result is accurate and intuitive.
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