What cells and uses do 1.1601, 1602, 1603, and 1604 correspond to?
answer:
| Common cell lines & tumor cell lines | Stem cells & primary cells | |
| Serum-free DMSO | 1601 | 1602 |
| Serum-free and DMSO free | 1603 | 1604 |
2. How suitable is the cell cryopreservation density?
A: For most cell types, 1~3×106/ml can be used; for blood cells, higher density is needed, about 5×106/ml is recommended.
3. How many cells are stored in each cryotube?
A: No more than 1 ml of cell suspension should be used in each cryotube. Otherwise, the rewarming time will affect the cell resuscitation effect.
4. Does LiveCyte need to cooperate with the program cooling step or program cooling device?
A: No. LiveCyte is specially developed for direct and rapid freezing of -80 ° C low-temperature refrigerator. A variety of protective agents in the frozen solution can avoid damage to the cells; if the program is used to cool the box, it is not conducive to cryopreservation. You can freeze the cells at -80 °C for 24 hours and transfer the cells to a liquid nitrogen tank for long-term storage.
5. Can LiveCyte cryopreserved cells be stored directly in liquid nitrogen?
A: No. Suitable for direct liquid nitrogen cryopreservation technology called vitrification, LiveCyte's existing product formula is not suitable for vitrification. We can provide special products according to your needs to meet your various practical needs.
6. Which cells can be frozen using LC1601?
A: LC1601 cryopreservation can be used in most types of cell lines and cell lines; please select the appropriate product according to the characteristics of your frozen cells. To ensure that your cells are suitable, you can pre-freeze the test once in advance. If you are not sure which product to choose from, you can contact us and we will help you choose the most suitable cryopreservation solution.
7. Which cells can be stored using LC1602?
A: Primary cells, stem cells or other cells that are not easily frozen by tissue; LC1601 is also suitable for LC1601.
8. Which cells can be cryopreserved using LC1603 and LC1604?
A: Cell types that are sensitive to DMSO components and affect resuscitation efficiency, such as islet cells; cell types that have higher requirements for cell differentiation, such as mesenchymal stem cells.
9. Does the LiveCyte series contain serum components?
A: All LiveCyte products are serum free, so you don't have to worry about serum.
10. Does LC1601 and LC1602 contain DMSO?
A: Contains. Some cells are sensitive to DMSO, and we have developed LC1603 and LC1604 without DMSO.
11. What is the difference between LC1601 and LC1602?
A: LC1602 has more humanized cryoprotectant protein and a reducing agent for the survival of beneficial cells than LC1601, which can better protect precious cells.
12. Can LiveCyte cryopreserved cells be stored for a long time?
A: Cryopreserved cells using the LiveCyte series significantly extend the storage time of -80 degrees. If longer storage is required, it is recommended to move to liquid nitrogen.
13. Which cryopreservation solution should hematopoietic stem cells, mesenchymal stem cells and PBMC use?
A: It is recommended to use LC1602 cryopreservation solution.
14. Which cryopreservation solution should ES cells and iPS cells use?
A: You can use LC1602 for cryopreservation. The company is currently developing the special cryopreservation solution LiveCyte-Stem for human ES and iPS, which can significantly improve the cell recovery rate and is expected to be available in early 2017.
15. What is the chemical composition of serum-free cryopreservation solution, does it affect cell function and differentiation?
Answer: The LiveCyte series of cryopreservation solution is composed of high molecular safety organic matter, glucose, sodium bicarbonate, HEPES buffer and DMSO. It has clear chemical composition and no heterogeneous animal components. It is safe and reliable. No observation is made. Cell function and differentiation potential have an impact.
16. What are the reasons for the poor performance of LiveCyte cryopreservation?
A: The LiveCyte series of cell cryopreservation solution has a good cryopreservation effect on most commonly used cells. If the cell cryopreservation is not ideal, it should be analyzed specifically. The most common cause is that the cell has mycoplasma contamination. In this case, Regardless of the cryopreservation method, the cell viability after resuscitation is very low; the second is that the temperature of the refrigerator in which the cells are stored does not reach -80 degrees, or the temperature of the refrigerator is unstable, and the opening time is longer; the third is not after the cell recovery Washing the cells with the medium resulted in more residual DMSO, resulting in a decrease in cell resuscitation.
17. What is the use of similar products abroad?
A: At present, LiveCyte's similar products have been widely used in foreign laboratories. For example, in the Japanese market, 80% of laboratories have used similar products for routine cell cryopreservation.
18. Can LC1602 and LC1604 cell cryopreservation be used to cryopreserve tumor tissue?
A: No, please use LiveTissue to freeze the tumor tissue.
Shanghai Keyuandi Biotechnology Co., Ltd.
Http://
Telephone / Cell phone
QQ
WeChat mail:
Food additives are compounds or natural substances added to food for the purpose of improving the color, aroma, and taste of food, as well as for the needs of anti-corrosion and processing technology. At present, there are 23 categories of food additives, with more than 2,000 varieties, including acidity regulators, anticaking agents, defoamers, antioxidants, bleaching agents, leavening agents, coloring agents, color retention agents, enzyme preparations, and flavor enhancers, nutritional fortifiers, preservatives, Sweeteners, thickeners, spices, etc.
Erythritol, Sweetener, Pigment, XOS , Carotene, fish oil
Xi'an Gawen Biotechnology Co., Ltd , https://www.ahualyn-bios.com