(1) Reduce the number of freeze-thaw cycles as much as possible.
(2) The culture medium does not require a 37 ° C water bath.
(3) The medium is kept at a pH of 7.0-7.2.
(4) Strictly control the water quality of the preparation medium, and the container should be brushed regularly.
(5) Master the timing of cell passage, and the cells should not grow too old.
1. Freeze the serum from the -20 degree refrigerator and freeze it at room temperature (or immersed in tap water) (about 30 minutes to 2 hours, or put it in a 4 degree refrigerator for overnight freezing; if it is not immediately inactivated after thawing, Put it in a 4 degree refrigerator for temporary storage)
2, inactivate
(1) Start heating in a water bath at room temperature (put in an empty serum bottle, fill 100ml of tap water, insert a thermometer, temperature monitoring)
(2) When the thermometer shows 56 degrees, stop heating and change to intermittent heating, maintaining 56 degrees (±0.5 degrees) for 30 minutes (±3 minutes; if the temperature rises more than 56 degrees, if it still does not exceed 60) Degree, and the time is very short, such as no more than 5 minutes, it can still be used; if it is more than 60 degrees, or longer, discard it, or try to cultivate some common cells)
(3) Take out and naturally cool to room temperature (about 1-3 hours). It can be stored separately or at -20 degrees.
3. Packing
(1) Transfer to the sterile room, and place the serum into a 10ml centrifuge tube in the clean bench (note that the serum should be gently shaken for several weeks before mixing; when using a pipette or a large tube to blow out the serum) Note: Do not blow out bubbles (the serum is very viscous and it is easy to produce bubbles; if bubbles are generated, it will be on the flame of the alcohol lamp))
(2) Store in a -20 degree freezer
(3) All operations take about 4-6 hours
Fluorine-Containing Phenylamine
Most of the Fluorine-Containing Phenylamine products are a light yellow oily liquid, relatively high density, insoluble in water. Most of them are used in the manufacturing of pesticides and dye intermediates, small part of them can be used as an analytical reagent. The steam or smoke of fluorine-containing phenylamine series is irritated to eyes, mucous membranes and upper respiratory. Vapor and air can form explosive mixtures, in case of fire, high-heat combustion caused the explosion. And oxidant may react. Decomposition by high fever and emit toxic gases. In case of high fever, increased pressure within containers, cracking and the risk of explosion. Protective measures must be done carefully during product storage. Once the fire occurred, it must be immediately evacuated from air leakage,personnel to a safe area, prohibit access to the contaminated area. Recommended emergency personnel wearing self-contained breathing apparatus and wear chemical protective clothing. In the ambulance personnel to ensure proper safety measures, immediately use foam, carbon dioxide, dry, sandy soil to put out a fire.
Containing Phenylamine,2-Bromo-4-Methoxy-Phenylamine,5-Fluoro-2-Iodoaniline
Taizhou Volsen Chemical Co., Ltd. , https://www.volsenchem.com