The His tag is a short peptide consisting of six histidines (His-His-His-His-His-His) specifically designed for the adsorption purification of recombinant proteins. Due to its small molecular weight and its ease of isolation and purification, His-tag fusion tags have many advantages over other tags and are the most widely used fusion tags currently used for purification. His antibody can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins. However, currently commercialized His-tag monoclonal antibodies (mAbs) are rare in variety and expensive.
How to choose the appropriate His tag antibody?
When choosing His antibodies, we mainly need according to our own needs, especially for experimental applications. Among the factors to be considered are the type of detection of His antibody, the clonality of His antibody, and the source of preparation. For example, can the His antibody be used for Western Bloting? Can it be used for immunofluorescence IF experiments? Do I need a monoclonal His-tag antibody with better specificity? Do I need to consider whether to choose a mouse-derived His antibody from a mouse source?
Previously, there was a His-tagged antibody using SSS (a famous brand of His-tagged antibody, the name was hidden). It was good at the beginning. After half a year, the product quality was unstable. It was not possible to do an IF experiment. Later, I tried Abbkine. The results of IF are very beautiful. By the way, they also bought their DyLight fluorescent secondary antibody - a customer of Guangzhou Southern Medical University.
In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources generally have better specificity, stability and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100μl His antibody (if the dilution ratio of WB is 1:5,000, the final solution is equivalent to 500ml), 1ml of His antibody is lower than the titer (if the dilution ratio of WB detection is 1:200) The final use solution is equivalent to 2ml). Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.
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| Product name and description | Brand | Item number | Product Details |
| Anti-His Tag Mouse Monoclonal Antibody (5C3) | Abbkine | A02050 | Details |
| HRP Conjugated Anti-His Tag Monoclonal Antibody (5C3) | Abbkine | A02055 | Details |
| Anti-His Tag Rabbit Polyclonal Antibody | Abbkine | A02051 | Details |
Why recommend Abbkine's His antibody?
There are many imported brands that supply His antibodies, including Sigma, Novagen, Qiagen, Abcam, etc. Among them, Abcam has a recommended His antibody, called 6X His tag® antibody [HIS.H8] (Cat. No.: ab18184), the antibody It is the only 5-star His-tag antibody recommended by Abcam for IF/ICC, IP and WB experiments. As of 2013, there are 16 articles published in NCBI using this antibody (for details, please click: 6X His tag® antibody [HIS.H8]). The antibody is a monoclonal antibody, the clone number is HIS.H8, and the 2013 price of this antibody in China is ¥4418/100 μl. Abcam's antibodies are highly recommended in terms of brand awareness, quality and after-sales, and then the price is higher.
Here, we recommend Abbkine's His-tag antibody, Anti-His Tag Monoclonal Antibody (5C3) (Cat. No. A02050), which is actually the same source as the above Abcam antibody. Like Abcam, the immunogen is a 6x His synthetic polypeptide that recognizes His-recombinant proteins and His-tagged recombinant proteins that are overexpressed in cells. However, its price is 65% lower than Abcam. As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. The antibody can be used in a variety of different applications such as Western Bloting, immunocytochemistry and immunofluorescence ICC/IF and immunoprecipitation IP assays.
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| Product name and description | Brand | Item number | Product Details |
| Anti-His Tag Mouse Monoclonal Antibody (5C3) | Abbkine | A02050 | Details |
| HRP Conjugated Anti-His Tag Monoclonal Antibody (5C3) | Abbkine | A02055 | Details |
| Anti-His Tag Rabbit Polyclonal Antibody | Abbkine | A02051 | Details |
A high titer antibody not only affects the sensitivity of the experimental results, but also means a higher price/performance ratio. Abbkine's His-tag antibody In the conventional WB experiment, the recommended initial dilution ratio is 1:1000. Actually, in Abbkine application, there is a dilution ratio of 1:4,000 or higher, which needs to be based on the customer's specific experimental conditions. optimization. The antibody is also excellent in immunocytochemistry and immunofluorescence ICC/IF, and the immunological determinant recognized by His-tag antibody of 5C3 clone is very suitable for ICC/IF application. IP immunoprecipitation is also an important reason for customers to choose Abbkine. In addition, Abbkine also provides HRP-conjugated His-tag antibody, which is especially suitable for the removal of secondary antibody interference during IP-WB verification. Rabbit-derived polyclonal His antibody also provides customers with another His-tag detection protocol.
Abbkine also offers a large package of 1ml His-tag antibody, which is ideal for the experimental needs of the immunoassay of the His-tag system. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.
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| Product name and description | Brand | Item number | Product Details |
| Anti-His Tag Mouse Monoclonal Antibody (5C3) | Abbkine | A02050 | Details |
| HRP Conjugated Anti-His Tag Monoclonal Antibody (5C3) | Abbkine | A02055 | Details |
| Anti-His Tag Rabbit Polyclonal Antibody | Abbkine | A02051 | Details |
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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