The insect antennae potential measurement system is used to record changes in insect antennae potential when different stimuli are applied, and to study the electrophysiology of insects, which are widely used in research fields such as plant protection, biological control, and forest diseases.
The EAG insect antennae potential measurement system is widely used as a biometric instrument in experimental entomology to detect insects that perceive the world through antennae. It is recorded between the tip and root of the insect antennae under the action of external hormones. A small voltage fluctuation, the amplitude of the voltage value becomes larger as the concentration of the stimuli increases, and it is known that the saturation state is reached. The change in amplitude is more dependent on the type of stimulus, the type of insect, gender, and other factors.
At present, EAG technology has been applied in many aspects. For example, screening active compounds, purifying extracts, selecting active compositions, and the like. The following describes several measurement systems related to insect antenna potential.
EAG insect antennae potential measurement system According to the type of antenna, connect the insect antenna to the appropriate holder and connect it to carry out the experiment. The pre-tested stimuli sample is prepared, loaded into a Pasteur tube, and the irritant is blown toward the antennae by triggering the airflow control unit. The system belongs to a simple level of galvanic potential detection, which can detect whether the sample has a stimulating effect on the antennae, such as odor substances collected from green leaves, and detecting the reaction of an insect to the odor substance, which is qualitative detection and cannot judge the reaction. Specific substances. |
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GC-EAD Gas Chromatography Insect Antenna Potential Measurement System Compared with EAG, GC-EAD has further deepened the study of the antenna potential. The system consists of two major components: a gas chromatograph (recommended Agilent 7890/7890B) and an insect antennae potential measurement system. After the sample is separated by a gas chromatographic column, the gas component is divided into two parts, one part enters the gas chromatographic FID detector, and the other part is mixed with clean air and blown toward the antennae. Thus, the peak of the gas chromatograph can correspond to the peak of the EAG, and it is not necessary to extract each component to know which component is biologically active, and the component without the EAG reaction is excluded. |
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SSR insect antennae single sensor measurement system The SSR insect antennae single sensor measurement system can distinguish the response of different sensory cells and record the action potential of specific nerve cells. The amplitude of the action potential response of specific nerve cells is the same. As the activity of the compound increases, the frequency of the action potential reaction is excited. The higher, it is an effective complement to EAG and GC-EAD. The single cell reaction recording device is basically similar to the EAG reaction recording device except that the design of the amplifier is slightly different. Single cell responses include alternating nerve impulse responses and DC sensitized potentials, which differ in the same frequency band. The passband of the SSR is about 40-500 Hz, and the EAG is about 0-40 Hz. The auxiliary tungsten wire electrode is inserted into the interior of the insect eye, and the main tungsten wire electrode is inserted into the base of the hair sensor, and the nerve pulse reaction and the susceptor potential are obtained through high-pass and low-pass filtering of the software. SSR was used to study Daphnia sinensis D. In the hair styling sensitive hair sensor, each hair sensor usually has several olfactory sensory cells, and each olfactory sensor cell is sensitive to only one compound, and the sensitivity of the reaction is high, and the odor stimulation amount is high. At 10 -4 -10 -3 ug, it can cause obvious reaction. |  |
 | The dose-dependent response of Aedes albopictus olfactory receptor (AalOR2) to sputum increased from 10 -4 M to 2X10 -2 M, and the frequency of spike was directly proportional to the concentration of sputum, indicating that Specific response of mosquito olfactory receptors. The same reaction is plotted as shown below. |
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TasteProbe Insect Taste Measurement System The insect taste is mainly distributed in the antennae, the lower beard, the lower lip, the tongue, the inner lip, the pharynx, the foot and the ovipositor. Each taste sensor is distributed with taste neurons that sense different stimulating substances. When the stimulating substance enters the interior of the insect taste receptor, the taste receptor in the sensory neurons in the sensor can "encode" the stimulation information of the external chemical substance, convert the chemical signal of the stimulant into an electrical signal, and pulse through the axon. The form is transmitted to the central nervous system for integration, and then the central nervous system outputs instruction information for regulating behavior, and the stimulated insect completes the chemical reaction to the stimulating substance, and the application is widely used in research fields such as plant protection, biological control, and forest diseases. |  |
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