Nature Sub-Journal: Fighting Cancer, Try 3D Cell Culture Technology

Release date: 2015-11-11

Malignant tumors have become one of the leading causes of death in China, and early diagnosis and effective treatment of tumors have become the focus of life science research in various countries. The journal Nature Medicine recently published a paper on the new results of using three-dimensional cell culture technology to amplify and culture primary cancer cell organs from pancreatic cancer patients. This technology allows researchers to directly use patient tissue for targeted research and rapid and economical individualized drug testing.

The author of the paper, Senthil Muthuswamy, is a professor at Harvard Medical School and director of the Cell Biology Division at the Cancer Research Center of the Beth Israel Deaconess Hospital. He said, "These three-dimensional organs are essentially tiny tumors that grow in petri dishes. They retain the characteristics and functions of the patient's tumor cells and can therefore serve as a new platform for tumor research and treatment."

This technique was completed during his research at the University of Toronto, Canada. The first author of the paper is Dr. Huang Ling. Several researchers and doctors at the Ontario Cancer Institute participated in the study. Professor Muthuswamy and Dr. Huang Ling continue the study at Harvard Medical School.

In the past two decades, scientists have begun to use three-dimensional cell culture techniques to study animal tissue development or tumor growth. As one of the first pioneers of this technology application, Professor Muthuswamy's laboratory used cell lines to study breast development and breast cancer dating back 15 years. The most important feature of this time's technological innovation is the ability to directly use the patient's own tissue rather than the experimental cell line for organ-like organ culture, and these organs can well preserve the pathological morphology and biological mechanism of the patient's tissue. In this study, the researchers also found that tumor-like organs can retain the sensitivity of the patient's tissue itself to new agents in vitro.

Professor Muthuswamy said, "From a research perspective, this method of culturing tumor-like organs enables us to effectively establish a living tissue bank of organisms. This library of living tissues can be used to discover and validate new drugs, simulations and Study the resistance of targeted drugs. If we use a group of patients' tumor-like organs to study the relationship between drug reactions and genetic mutations, we may be able to understand why some patients can respond effectively to the drug while another patient cannot Effective response to treatment. As a result, we are able to avoid unnecessary and ineffective treatments for patients. From a clinical perspective, this approach can help patients and physicians choose treatment options more effectively."

Peter Paolo Pandolfi, a professor at the Beth Israel Deaconess Hospital Cancer Research Center and a professor at Harvard University, commented on the study, "Pancreatic cancer is a very malignant disease. The survival rate of patients with advanced patients and tumors spread is only 3%. This requires us to have early diagnosis and new treatments. This type of organ culture technology allows us to establish a pancreatic cancer model to study and screen drugs for precise treatment."

Source: Medical Valley

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