Jilin Academy of Agricultural Sciences uses the pollen tube pathway method to introduce GNA gene into soybeans and screen genetic engineering bodies in two ways: First, adopting pollen tube channel technology to obtain seeds, sowing in artificially controlled sheds, inoculating aphids, and screening for resistant plants. After PCR identification and Southern blot hybridization identification; Second, the obtained seeds were first planted in pots, selected strains for PCR detection, identification of PCR-positive larvae; tested T1, T2, T3 PCR identification results confirmed that the target gene has been Integration into the receptor soybean genome. Southern blot also gave positive results. The results of the resistance to aphid identification showed that the transgenic plants were 50% more resistant than the control. It has successively selected and produced varieties such as “Jike Dou No. 1†and “Jinong No. 9â€.
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