1. The cultivation period of Tremella in the cultivation season is 35 to 45 days, and the mycelial growth stage is 15 to 20 days. The cultivation of Tremella is in spring and autumn, and the temperature is stable at about 25 ℃. In order to increase the turnover rate of the use of white fungus in suitable seasons, a two-zone system can be adopted, namely the germ room and the ear chamber. Five days before the first batch of white fungus is harvested, the second batch of white fungus will be bagged, inoculated and placed for germination. Indoor cultivation.
2. The production methods of various strains of white fungus are different from those of common edible fungi, so special introduction is needed. Because of the following characteristics of Tremella fungus hyphae: it cannot degrade lignocellulose in natural materials, cannot grow in sawdust medium, and grow slowly. It only grows around the ear base or within a few centimeters of the inoculation site, away from the ear base and inoculation site. Without Tremella hyphae, Tremella fungus hyphae are easy to kink and gelatinize to form primordia (ear buds). They will not die in 2 to 3 months in a silica gel dryer. They are not resistant to humidity and are easy to be used on sloping media with condensed water. Spore formation. Therefore, the separation of white fungus strains is mainly to take the material around the ear base and the inoculation site, and place it in a silica gel desiccator for 2 to 3 months, then take a small piece and transfer it to the PDA slope, and incubate at 22℃～25℃ for 10-15 days. White fungus hyphae were obtained.
3. The production of fungus bag takes cotton seed hull as the main cultivation material, and wheat bran and gypsum as auxiliary materials. Cotton seed husks require good air permeability, rich nutrition, comprehensiveness, and no mold. Wheat bran provides rich nitrogen nutrition for the white fungus, and also provides necessary vitamins. Fresh, mildew-free wheat bran should be used. In addition, gypsum can be added, either raw gypsum or paris gypsum, and it is required to be powdery for easy mixing.
For Tremella cultivation, polypropylene plastic bags are used. Each bag can contain 600 grams of dry material. The amount of various raw materials is calculated according to the production bag. According to the formula, cotton seed hulls are 83%~90%, wheat bran 14%~17%, and gypsum 2%. The ratio of material to water (1:1.2) is to push the cottonseed husk away on a clean cement floor, sprinkle wheat bran on the cottonseed husk, and finally sprinkle the gypsum. Add clean water in an amount 1.2 times the weight of the dry material, use a semi-automatic screw press-feeding machine to fill, and fill the culture material to about 8 cm from the bag mouth. Wipe clean the adhering culture material on both sides of the bag mouth, tying it with cotton thread for 3 times, then fold it, then re-tie it for 2 times, and then tighten the thread.
4. Ear management 9-12 days after inoculation, the colony diameter is 8-10 cm, white with black spots. At this time, move the bacteria bag in and out of the ear chamber and discharge it on the bed frame, and lightly spray water 1 to 3 times a day to keep the tape moist. Control room temperature 22℃~25℃, relative humidity 75%~80%. Pay attention to ventilation, ventilate 3 to 4 times a day, 10 minutes each time, and maintain low light. 13-16 days after inoculation, the hyphae are almost covered with bacteria bags, yellow water drops appear in the holes, tear off the tape, cover the woven fabric, spray water for humidification, lift the cloth for oxygenation 1 to 2 times a day, and control the room temperature at 22°C-25°C. The relative humidity is 85%～90%. Ventilate 3 to 4 times a day, 20 minutes each time, with the mouth of the bacteria bag facing sideways, allowing the yellow water to flow out.
17-19 days after inoculation, the light yellow primordium is formed, and then the primordium differentiates into ear buds. At this time, it is required to cut the membrane 1 cm, spray water on the cloth to keep it moist, control the room temperature at 20°C to 22°C, and take the temperature rise and fall in due course. Measures. Control the relative humidity from 90% to 95%, and ventilate 3 to 4 times a day for 30 minutes each time.
20-25 days after inoculation, the flowers are 3-6 cm in size, the ears are not spread, and the color is white. At this time, it is required to take the cloth and dry it before covering it, and spray water to keep it moisturized. The room temperature is controlled at 20℃～24℃, and the relative humidity is 90%. ~95%, ventilate 3 to 4 times a day, 20 to 80 minutes each time, spray more water on the ears and less water on the ears, combined with ventilation, increase the scattered light.
26 to 30 days after inoculation, the size of the flowers is 8-12 cm, the ears are loose, and the color is white. At this time, open the non-woven fabric and spray water, control the room temperature 20℃~24℃, relative humidity 90%-95%, and ventilate 3 times a day. ～4 times, 20-30 minutes each time, mainly wet, alternating dry and wet, spray more water on sunny days, combined with water spray and ventilation.
31~35 days after inoculation, the size of the flowers is 12~16 cm, the ears are slightly shrunk, the color is white, the base is yellow and elastic. At this time, stop spraying water, control the temperature, and wait to be harvested. Control the room temperature to 20°C to 23°C, the relative humidity to 80% to 85%, and ventilate 3 to 4 times a day, 30 minutes each time, 35 days later, choose sunny harvest.
5. Preventive measures should be taken to prevent pests and diseases, improve the environmental sanitation of cultivation sites, create an environment suitable for the growth of tremella hyphae and fruit bodies, and reduce the incidence of pests. If the damage of Alternaria, Trichoderma viride, rotten ears, and "Yellowberry Frost Disease" is found, the damaged tube should be packed in a clean and sealed plastic bag and removed from the cultivation site in time. The less harmful ones can be re-sterilized and reused. , The more serious ones are directly incinerated or buried. At the same time, it is necessary to strengthen ventilation, prevent high temperature and high humidity, appropriately reduce humidity, and stop spreading.
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