In recent days, rapeseed growers have reacted and field rape seedlings have developed diseases. The main symptoms are root swelling, pale leaf color in the lower leaves of plants, and severe yellowing and wilting. This is a typical symptom of clubroot disease. The incidence of this disease in the main areas of rapeseed production and rape cultivation in China has increased year by year in recent years and should be paid attention to.
The clubroot disease generally invades from the root hair or young roots and grows and develops first in the root hairs. This stage generally does not show symptoms. When the disease continues to develop and invade the cortex tissue and the formation layer of the roots, the host cells are stimulated, the division is accelerated, and the volume increases. Due to the mutual extrusion between cells, the vascular bundle tissue is not normally developed, and the roots are swollen. Severe diseases at the seedling stage can be withered.
Causes of the disease: First, as the root edema bacteria in the soil with the roots of the winter, summer, and can survive in the soil for more than 10 years, and disease-causing rapeseed cultivation for a long period of continuous cultivation, to provide a very favorable conditions for the propagation of bacteria. The second is the long-term use of chemical fertilizers, pesticides, etc., and no soil improvement, resulting in excessive soil acidity.
Prevention and control measures: The clubroot disease is a soil-borne and species-borne disease. Once the disease is introduced, single control technology is difficult to control effectively. Comprehensive prevention measures are recommended.
1. Select resistant varieties and implement rotation. Planting resistant varieties is the most effective disease prevention measure. 5 years or more rotation with non-cruciferous crops.
2. Adjust soil pH. Mu 100-150 kilograms of slaked lime are evenly spread on the surface of the soil, and are fully mixed in the soil by the site preparation.
3. Remove and destroy diseased plants in time. The diseased plants were found, removed in a timely manner, and fired at high temperatures or dried and then uniformly burned. The diseased plants must not be left in the fields or thrown in other areas to prevent the spread of larger areas of the bacteria.
4. Chemical control. Rinse rape seedlings with 1500-2000 times solution of Kejia Suspending Agent and water around the seedlings (within 15-20 cm in diameter) (to be thoroughly drenched and reach a depth of 15 cm), requiring 250 ml per seedling. The amount of liquid medicine is better for the early stage of rape seedlings.
The kit is used for in vitro qualitative detecting the ORF1ab/N genes of novel coronavirus 2019-nCoV and Delta Variant in respiratory specimens including oropharyngeal swabs, nasopharyngeal swab, sputum and bronchoalveolar lavage fluid, and performing D950N mutation detection of Delta variant of Novel Coronavirus 2019-nCoV.
As reported in GISAID database, more than 95% Delta variant of Novel Coronavirus 2019-nCoV carry D950N mutation.
Primer sets and FAM labeled probe are designed for specific detection of ORFlab gene of 2019-nCoV, VIC labeled probe for N gene of 2019-nCoV, ROX labeled probe for D950N mutation of Delta variant. Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with CY5.delta test reagent,delta variant test reagent,delta variant reagent,delta test kit,delta reagent kit
Shenzhen Uni-medica Technology Co.,Ltd , https://www.unimed-global.com