At present, with the gradual implementation of national food safety regulations, more and more microbial testing laboratories in China are paying more and more attention to the enrichment culture of microaerobic bacteria, and the cultivation equipment for microaerobic bacteria is gradually paid more attention. A practical and easy to operate micro-aerobic workstation will be elaborated in this guide.
1. Oxygen concentration requirements of microaerobic bacteria:
Defined in Chapter 7 "Microbial Growth" in the Microbiology textbook of the University of Georgia, USA: Microaerobic bacteria are a group of bacteria that grow at low oxygen levels (2~10%), usually with atmospheric oxygen levels. .
2. The oxygen concentration control range of the micro-aerobic workstation:
In order to ensure the good growth of microaerobic bacteria, the oxygen concentration control range of the micro-aerobic workstation should be 3~8%.
3, micro aerobic workstation classification:
A, oxygen sensor dependent micro-aerobic workstation
Such a workstation is provided with an oxygen concentration sensor in the inner cavity, through which the oxygen concentration of the inner cavity is detected in real time, and the signal value is transmitted to the controller, and the compressed air cylinder intake valve is adjusted by the controller, thereby realizing the internal working The micro-aerobic environment of the cavity is regulated.
B, non-oxygen sensor dependent micro-aerobic workstation
This type of workstation is a positive pressure chamber designed for mechanical forced directional airflow. Mechanical forced convection ensures lumen gas uniformity, while positive pressure feedback regulation utilizes gas replacement during operation to ensure a lumen oxygen concentration environment and a microaerobic mixture ( The 5% O2+10%CO2+85%N2) cylinders are identical, thus achieving a micro-aerobic environment regulation of the working cavity.
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