Rat (PAL) ELISA kit technology principle
Rat L-phenylalanine ammonia-lyase (PAL) ELISA kit rat L-phenylalanine ammonia-lyase (PAL) ELISA kit technology supports rat L-phenylalanine ammonia-lyase (PAL) ELISA kit Manual Qiao Yu biological stock supply a variety of species, various brands Elisa kit, ELISA kit technology principles, ELISA analysis kit instructions, ELISA kit technical information, short delivery time, excellent quality, national free home delivery Jiangsu, Zhejiang and Shanghai can be reached the next day, and the province needs 3 to 5 days. Qiao Yu Bio Elisa kit is a big summer promotion, the offer is non-stop, welcome new and old customers to call to order!
Product Name: Rat (PAL) ELISA Kit Technology Principle
English name: Rat L-Phenylalanine ammonla-lyase, PAL ELISA Kit
Product No:QY-SZ2076
Product traits: liquid
Product use: dedicated to scientific research
Product specifications: 96T/48T
Customer Service Phone: 021-51867124 QQ Email @qq.com
ELISA kit composition and reagent preparation (the following is the general instructions for ELISA kits, does not include special kits, please refer to the instructions for each product for specific instructions!)
1. Enzyme plate: one piece (96 holes)
2. Standard (freeze-dried): 2 bottles, each bottle is diluted to 1ml with the sample dilution before use, and then allowed to stand for more than 10 minutes, then repeatedly reversed/twisted to help dissolve. The concentration is 1,600pg. /ml, after diluting it to 400 pg/ml, do a series of dilutions (note: do not directly dilute in the plate), diluted to 400 pg / ml, 200pg / ml, 100 pg / ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, the sample dilution was directly prepared as a standard concentration of 0 pg/ml, and was prepared within 15 minutes before use. For example, prepare 200 pg/ml standard: take 0.5ml (not less than 0.5ml) 400 pg/ml of the above standard into an Eppendorf tube containing 0.5ml of sample dilution, mix well, and so on.
3. Sample dilution: 1 x 20 ml.
4. Test dilution A: 1 x 10 ml.
5. Detect dilution B: 1 x 10 ml.
6. Detection solution A: 1 × 120μl (1:100) diluted with the test solution A 1:100 before use, prepared according to the total amount of each experiment required before dilution (100μl / hole), the actual More 0.1-0.2ml should be prepared during preparation. For example, 10 μl of test solution A plus 990 μl of test diluent A was prepared, gently mixed, and prepared within one hour before use.
7. Detection solution B: 1 × 120 μl / bottle (1: 100) diluted before use to detect dilution B 1:100. The dilution method is the same as the detection solution A.
8. Substrate solution: 1 x 10 ml / bottle.
9. Concentrated washing solution: 1 × 30ml / bottle, each bottle is diluted 25 times with distilled water.
10. Stop solution: 1 x 10 ml / bottle (2N H2SO4).
11. Lamination: 5 sheets
12. Instruction manual: 1 copy
Bring your own items
1. Microplate reader (recommended to refer to the instrument instructions for preheating)
2. Micro dosing device and tip, EP tube
3. Distilled or deionized water, new filter paper
Collection and preservation of specimens
1. Serum: Whole blood samples should be placed at room temperature for 2 hours or 4 °C overnight, centrifuged at 1000 xg for 20 minutes, the supernatant can be detected, or the specimens should be stored at -20 ° C or -80 ° C, but should be avoided. Freezing and thawing.
2. Plasma: EDTA or heparin can be used as anticoagulant. Centrifuge at 2 - 8 ° C 1000 xg for 15 minutes within 30 minutes after collection, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing. melt.
3. Other biological specimens: centrifuge at 1000 xg for 20 minutes, take the supernatant for testing, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing and thawing.
4. Sample processing: Serum or plasma specimens are recommended to be diluted 5,000 times.
Note: The above specimens should be stored at 4 ° C for less than 1 week, -20 ° C or -80 ° C should be sealed and stored, -20 ° C should not exceed 1 month, -80 ° C should not exceed 2 months;
After the collection of pathogenic bacteria specimens, the disposable sampling cotton Swab should be completely inserted into the preservation solution to maximize the retention of pathogenic bacteria.
The collected specimens must be fresh and sent for inspection in time to avoid the death of pathogenic bacteria.
It is forbidden to use products with damaged packaging and expired expiration dates to prevent contamination.
Flexible combination: the number and type of swabs and sampling tubes can be combined arbitrarily.
Wide range of uses: It can be used for the collection, transportation and storage of new coronavirus, respiratory virus, hand, foot and mouth virus and other specimens.
Excellent quality: It has been verified by many medical institutions that the virus can still be detected after being stored in the preservation solution for 7 days at room temperature.
Easy to distinguish: the inactivated and non-inactivated preservation solutions are pink, which can effectively avoid sample addition confusion.
Disposable Virus Sampler,Nasal Virus Sampler Throat Swab,Plastic Transport Tube For Saliva Collection,Viral Transport Tube With Swab
Jiangsu HXRT MD Co.,Ltd , https://www.jshxrtmed.com